Journal: Nature Communications

Article Title: Serotonin receptor 3A controls interneuron migration into the neocortex

doi: 10.1038/ncomms6524

Figure Lengend Snippet: ( a ) Microdissection of cortical tissue (dotted lines) containing GAD65 -GFP + INs was performed at E14.5 corresponding to the phase of tangential migration or at E17.5 during the phase of cortical invasion. GAD65-GFP + INs were platted in culture and time-lapse imaging was performed at day in vitro 1 (+DIV1). ( b ) Illustrative time-lapse sequence showing that GAD65 -GFP + INs (arrowheads) at E17.5 (+DIV1) increase their migration after exposure to the 5-HT 3A R agonist SR57227 (100 nM). Cells were tracked during a control period and a drug period of 360 min each. ( c , d ) Quantification revealed that 5-HT 3A R activation significantly increases the migratory speed and decreases the pausing time of GAD65 -GFP + INs at E17.5 (+DIV1; n =156 cells in three independent experiments; c ) but not at E14.5 (+DIV1; n =140 cells in three independent experiments; d ). *** P <0.001, paired Student’s t -test. Error bars are means±s.e.m. Scale bars: ( a ) 150 μm; ( b ) 10 μm.

Article Snippet: Following a control time-lapse imaging period of 360 min, SR57227 hydrochloride (100 nM; Tocris), serotonin (5-HT, 100 nM; Tocris) or vehicle (NBM) was added to the medium and recordings were performed for an additional 360 min.

Techniques: Laser Capture Microdissection, Migration, Imaging, In Vitro, Sequencing, Control, Activation Assay